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Collectively, the present results indicate that G4L1 is a promising biocontrol agent for tomato bacterial wilt. In the re-isolation experiment, G4L1 efficiently colonized foliar tissues for at least 4 weeks after spray application. A quantitative real-time PCR analysis revealed that the foliar spraying of tomato plants with G4L1 up-regulated the expression of PR-1a and LoxD in stem and GluB in roots upon the pathogen inoculation, implying that the induction of salicylic acid-, jasmonic acid-, and ethylene-dependent defenses was involved in the protective effects of this strain. Moreover, the pathogen population in the stem of G4L1-treated plants was markedly smaller than that in control plants. G4L1 displayed consistent and significant disease suppression, and, thus, was selected as a biocontrol candidate. In subsequent pot experiments, the biocontrol efficacy of foliar spray application was examined under glasshouse conditions. strains G1S3 and G4L1 significantly suppressed the incidence of tomato bacterial wilt. In the primary screening, we assessed the suppressive effects of a shoot-dipping treatment with bacterial strains against bacterial wilt on tomato seedlings grown on peat pellets. gloeosporioides), which significantly decreased disease incidence by 56 and 89, respectively, and reduced lesion diameter by 91 and 92, respectively. rhizophilaKM02 strain were the best antagonists to anthracnose (C. Some of the cell-free supernatants (CFS) tested showed in vitro antifungal activity versus pathogenic fungi. Twenty BCAs were tested against ten pathogenic fungi. The aim of this study was to carry out a screening of twenty biocontrol agents (BCAs) for their potential fungicidal activity in vitro. hansenii 1R11CB strain and marine bacteria S. Food bio-preservatives are requested as substituents of chemical pesticides in food. class of antimicrobial agents, constituting an unusual microbial weapon. When yeasts and bacteria were tested on mango fruit, the marine yeast D. Fifty heat-tolerant endophytic bacteria were isolated from the surface-sterilized foliar tissues of symptomless tomato plants that had been pre-inoculated with the pathogen Ralstonia pseudosolanacearum. strain B10 was the first bacteria showing biocontrol of plant.
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The aim of the present study was to identify a strain of endophytic Bacillus species that control tomato bacterial wilt by foliar spray application. Contrarily to fungal biocontrol agents, bacteria selected to biocontrol mango anthracnose come from a wide range of environments: plant isolate collections 35, soil 36, green banana surface 37 or mango leaf 38 39.